Primer

Part:BBa_K4810025:Design

Designed by: Theodoros Desiniotis   Group: iGEM23_Athens   (2023-10-11)


PCR reverse primer for modification of VOC-binding pVIII M13 protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 7
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primer contains PstI restriction site used to ligate digested M13KE vector to re-circularize


Source

Synthetic DNA

References